Journal: Acta Obstetricia et Gynecologica Scandinavica
Article Title: A novel multiple marker microarray analyzer and methodology to predict major obstetric syndromes using surface markers of circulating extracellular vesicles from maternal plasma
doi: 10.1111/aogs.15020
Figure Lengend Snippet: Principle of analysis of the extracellular vesicle (EV) Array. A customized antibody microarray is generated onto glass slides. EVs from unprocessed plasma or UC enriched EVs are added and EVs are captured during incubation. Step 2 is either performed using a cocktail of anti‐CD9, CD63 and CD81 to detect the total amount of EVs or by using anti‐PP13 to detect only placenta‐derived EVs. After Step 2 fluorescent streptavidin is added prior to scanning.
Article Snippet: The detection of Total EVs was conducted with biotinylated anti‐human antibodies to CD9, CD63, and CD81 (LifeSpan BioSciences, Lynnwood, WA, USA).
Techniques: Microarray, Generated, Incubation, Derivative Assay